When
You are sharing a colored asset for a web viewer or a visual review.
Then
Keep a GLB as the visual handoff.
A GLB can carry the surface, material settings, and texture images together. Flattening it to STL would discard much of what the reviewer needs to judge. Keep the source project too if someone may need to change the model later.
When
Your slicer or fabrication partner explicitly asks for an STL.
Then
Export an STL from a checked mesh.
Inspect the intended object rather than exporting every visible item in a GLB scene. Confirm orientation and physical dimensions after import, then look for holes, internal faces, disconnected parts, and walls too thin for the planned process.
When
The physical result needs color as well as shape.
Then
Check which color-capable format and workflow the recipient supports.
A standard STL will not reliably transfer a GLB's textures. Some workflows accept 3MF or other formats, but support for specific color and texture features varies. Confirm the receiving software and machine requirements before discarding the original GLB.